Lloyd S. Miller, M.D., Ph.D.
M.D., Ph.D. – SUNY Downstate Medical Center
Cancer Research Building 2, suite 205
Department of Dermatology
Department of Medicine, Division of Infectious Diseases
Department of Orthopedic Surgery
Department of Material Science and Engineering
Johns Hopkins University School of Medicine
1550 Orleans Street
Baltimore, Md. 21231
Phone: (410) 955-8662
Key words: Staphylococcus aureus, innate immunity, adaptive immunity, host defense, inflammation
My laboratory has focused on the investigation of innate and adaptive cutaneous immune responses against Staphylococcus aureus skin infections. Our laboratory has made key discoveries involving the role of IL-1β and the inflammasome as well as IL-17/Th17 responses that mediate neutrophil abscess formation and host defense against S. aureus skin infections. In addition, we recently discovered that S. aureus epicutaneous exposure promotes increased skin inflammation due to the activity of IL-36, which provides insight into how S. aureus skin colonization promotes skin inflammation in diseases such as atopic dermatitis. Our ongoing research projects involve the study of pattern recognition receptors (Toll-like receptors, NOD-like receptors, formyl peptide receptors) and other host innate immune mechanisms such as antimicrobial peptides and release of early proinflammatory mediators that provide the initial host defense against S. aureus and in S. aureus-induced skin inflammatory responses. In addition, we study how the innate immune response directs the subsequent adaptive immune response (Th1, Th2 and Th17 cells and antibody production) to help prevent recurrent S. aureus and methicillin-resistant S. aureus (MRSA) skin and soft tissue infections. This work has important clinical and translational relevance as community-acquired MRSA strains are causing severe skin infections in healthy people outside of hospitals and are becoming increasingly resistant to antibiotics, creating a serious public health threat.
In addition to our work in the skin, we also investigate the protective immune responses and novel antibacterial implant coatings to help treat or prevent staphylococcal biofilm-related infection such as orthopedic implant infections and subcutaneous device-related infections (such as infections of pacemakers and implantable cardioverter-defibrillators). These infections represent a devastating complication of indwelling devices and prostheses, leading to increasing morbidity, mortality and rising healthcare costs. To study these infections noninvasively and longitudinally over time, we have combined mouse models of implant infections with in vivo bioluminescence and fluorescence imaging in conjunction with µCT imaging and high resolution X-ray imaging. The results of this research will provide new insights into protective immune responses and novel treatments against orthopedic and other surgical implant infections.
Profile: Publications and Interests