Ben Larman

H. Benjamin Larman, Ph.D.
Ph.D. – Harvard—MIT
Post-doc – Scripps Research Institute

Assistant Professor
Division of Immunology
Department of Pathology
Johns Hopkins School of Medicine
Ross Research Building, Room 664B
720 Rutland Avenue
Baltimore, MD 21205

Phone: 410-614-6525

Lab Webpage


Key words:  immunoassay development, human immunology, precision immunopathology

The Larman laboratory develops and deploys new technologies to study the human immune system. Much of our work leverages advances in high throughput DNA synthesis and sequencing to enable novel, highly multiplexed molecular assays. Examples include:
– phage display of synthetic peptidome libraries for comprehensive profiling of antibodies;
– ribosome display of ORFeome libraries for antigen discovery, protein-protein interaction studies, and drug target identification;
– ultrasensitive, multiplexed RNA quantification techniques to monitor gene expression and detect microbes;
– efficient T cell receptor repertoire analysis;
– pooled genetic screening to elucidate immune cell function and identify new therapeutic targets.
The laboratory uses these and other unbiased approaches to identify new opportunities for monitoring and manipulating immune responses.

Profile: Publications and Interests

Selected Publications

Larman HB, Zhao Z, Laserson U, Li MZ, Ciccia A, Gakidis MAM, Church GM, Kesari S, LeProust EM, Solimini NL & Elledge SJ, “Autoantigen discovery with a synthetic human peptidome,” Nat. Biotech. 2011; 29(6): 535-41. PMID 21602805

Larman HB*, Salajegheh M*, Nazareno R, Lam T, Sauld J, Steen H, Kong SW, Pinkus JL, Amato AA, Elledge SJ & Greenberg SA, "Cytosolic 5′-nucleotidase 1a autoimmunity in sporadic inclusion body myositis," Ann. Neurol. 2013; 73(3): 408-18. PMID 23596012

Zhu J*, Larman HB*, Gao G, Somwar R, Zheng W, Laserson U, Ciccia A, Pavlova N, Church GM, Kesari S & Elledge SJ, "Protein interaction discovery using parallel analysis of translated ORFs (PLATO)," Nat. Biotech. 2013; 31(4): 331-4. PMID 23503679

Larman HB, Scott ER, Wogan M, Oliveira G, Torkamani A & Schultz PG, "Sensitive, multiplex and direct quantification of RNA sequences using a modified RASL assay," Nucleic Acids Res. 2014; 42(14):9146-57. PMID 25063296

George J. Xu, Tomasz Kula, Qikai Xu, Mamie Z. Li, Suzanne D. Vernon, Thumbi Ndung’, Kiat Ruxrungtham, Jorge Sanchez, Christian Brander, Raymond Chung, Kevin C. O’Connor, Bruce Walker, H. Benjamin Larman, & Stephen J. Elledge, “Comprehensive serological profiling of human populations using a synthetic human virome,” Science. 384(6239):aaa0698-1-9, 2015. PMID 26045439