Two projects are currently carried out in the lab. We continue the effort to understand the molecular mechanism of NF-B p50 biogenesis. We will combine mutagenesis and an in vitro system that simulates p50 generation in vivo to analyze and dissect the mechanism in detail. Based on the knowledge on NF-B processing, we have designed and generated a p105-based NF-B super repressor. Collaborating with labs in the U.S. and overseas, we are testing how well this super repressor inhibits varies NF-B isoforms in different tissues and diseases. The second project investigates angiotensin receptor-mediated signaling. We are interested in understanding how different dimerization status of angiotensin receptor (monomer, homodimer and heterodimer) affects downstream signaling targets. We plan to define and dissect the particular status of angiotensin receptor that triggers IL-1 production in monocytes and macrophages.

Tong Q, Zheng L, Lin L, Li B, Wang D, Huang C, Li D. (2006) VEGF is upregulated by hypoxia-induced mitogenic factor via the PI-3K/Akt-NF-?B signaling pathway. Respir. Res. 172(8):4797-803 [PubMed]

Tong Q, Zheng L, Lin L, Li B, Wang D, Li D. (2006) Hypoxia-induced Mitogenic Factor Promotes VCAM-1 Expression via PI-3k/Akt-NF-?B Signaling Pathway. Am. J. Respir. Cell Mol. Biol. 11(1):77-85 [PubMed]

Fu, D., Kobayashi, M., and Lin, L. (2004) A p105-based inhibitor broadly represses NF-?B activities. J. Biol. Chem. 4(4):291-9 [PubMed]

Lin, L., and Kobayashi, M. (2003) Stability of the Rel homology domain is critical for generation of NF-?B p50 subunit. J. Biol. Chem. 20(3):364-73 [PubMed]

Lin, L., DeMartino, G.N., and Greene, W.C. (2000) Cotranslational dimerization of the Rel homology domain of NF-?B 1 generates p50/p105 heterodimers and is required for effective p50 production. EMBO.J. 198 (1):31-8 [PubMed]

Heusch, M., Lin, L., Geleziunas, R., and Greene, W. C. (1999) The generation of nfkb2 p52: mechanism and efficiency. Oncogene 263:13901-13908 [PubMed]

Lin, L., DeMartino, G.N., and Greene, W.C. (1998) Cotranslational Biogenesis of NF-?B p50 by the 26S proteasome. Cell 143:4275-4281 [PubMed]

Lin, L., and Ghosh, S. (1996) A glycine-rich region in NF-?B p105 functions as a processing signal for the generation of the p50 subunit. Mol. Cell. Biol. 6:2047-2057 [PubMed]